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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has been noticed in lots of tumors, which ends up in chromosome instability, and thus may add to tumorigenesis. This protein was located to associate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in cell-cycle controlled histone gene expression and performs a essential part in endorsing mobile-cycle progression during the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, have been explained. Two additional splice variants ended up noted but detailed nucleotide sequence information and facts is just not nonetheless obtainable.
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Blocking peptides are peptides that bind particularly on the target antibody and block antibody binding. These peptide ordinarily incorporates the epitope recognized by the antibody.
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This guideline outlines further attainable optimization of mobile and immunoassay parameters to make sure the absolute best effects are...
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This guide outlines further more attainable optimization of mobile and immunoassay parameters to make certain the best possible final results are...
Antibodies certain to the blocking peptide no more bind to your epitope within the concentrate on protein. By evaluating the staining through the blocked antibody vs . the antibody on your own, you can see which staining is specific.
Focus on Information Cyclin E belongs on the highly conserved cyclin relatives, whose members are characterized by a spectacular periodicity in protein abundance through the cell cycle. Cyclins functionality as regulators of CDK kinases. Distinct cyclins exhibit distinctive expression and degradation styles which contribute towards the temporal coordination of every mitotic function. Cyclin E varieties a complex with check here and functions like a regulatory subunit of CDK2, whose action is needed for cell cycle G1/S changeover. This protein accumulates at the G1-S stage boundary and is also degraded as cells progress by way of S period.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by both equally antibodies...
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